In Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition, 19 (2015). For each decision tree, node importance is calculated using Gini importance, Eq. Experimental results have shown that the proposed Fuzzy Gabor-CNN algorithm attains highest accuracy, Precision, Recall and F1-score when compared to existing feature extraction and classification techniques. Extensive comparisons had been implemented to compare the FO-MPA with several feature selection algorithms, including SMA, HHO, HGSO, WOA, SCA, bGWO, SGA, BPSO, besides the classic MPA. Efficient classification of white blood cell leukemia with improved swarm optimization of deep features. In general, feature selection (FS) methods are widely employed in various applications of medical imaging applications. For more analysis of feature selection algorithms based on the number of selected features (S.F) and consuming time, Fig. We do not present a usable clinical tool for COVID-19 diagnosis, but offer a new, efficient approach to optimize deep learning-based architectures for medical image classification purposes. Latest Japan Border Entry Requirements | Rakuten Travel Accordingly, that reflects on efficient usage of memory, and less resource consumption. Memory FC prospective concept (left) and weibull distribution (right). the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Garda Negara Wisnumurti - Bojonegoro, Jawa Timur, Indonesia | Profil Accordingly, the FC is an efficient tool for enhancing the performance of the meta-heuristic algorithms by considering the memory perspective during updating the solutions. A comprehensive study on classification of COVID-19 on - PubMed Softw. 132, 8198 (2018). 152, 113377 (2020). Med. ADS Its structure is designed based on experts' knowledge and real medical process. Detecting COVID-19 at an early stage is essential to reduce the mortality risk of the patients. Automated detection of alzheimers disease using brain mri imagesa study with various feature extraction techniques. Generally, the most stable algorithms On dataset 1 are WOA, SCA, HGSO, FO-MPA, and SGA, respectively. Corona Virus lung infected X-Ray Images accessible by Kaggle a complete 590 images, which classified in 2 classes: typical and Covid-19. Etymology. Comput. Cancer 48, 441446 (2012). Cite this article. Covid-19-USF/test.py at master hellorp1990/Covid-19-USF Google Research, https://research.googleblog.com/2017/11/automl-for-large-scaleimage.html, Blog (2017). Support Syst. Med. Eng. Machine learning (ML) methods can play vital roles in identifying COVID-19 patients by visually analyzing their chest x-ray images. EMRes-50 model . Radiomics: extracting more information from medical images using advanced feature analysis. Highlights COVID-19 CT classification using chest tomography (CT) images. Bukhari, S. U.K., Bukhari, S. S.K., Syed, A. The test accuracy obtained for the model was 98%. The convergence behaviour of FO-MPA was evaluated over 25 independent runs and compared to other algorithms, where the x-axis and the y-axis represent the iterations and the fitness value, respectively. 2020-09-21 . They showed that analyzing image features resulted in more information that improved medical imaging. A NOVEL COMPARATIVE STUDY FOR AUTOMATIC THREE-CLASS AND FOUR-CLASS COVID-19 CLASSIFICATION ON X-RAY IMAGES USING DEEP LEARNING: Authors: Yaar, H. Ceylan, M. Keywords: Convolutional neural networks Covid-19 Deep learning Densenet201 Inceptionv3 Local binary pattern Local entropy X-ray chest classification Xception: Issue Date: 2022: Publisher: Boosting COVID-19 Image Classification Using MobileNetV3 and Aquila volume10, Articlenumber:15364 (2020) medRxiv (2020). Get the most important science stories of the day, free in your inbox. Dr. Usama Ijaz Bajwa na LinkedIn: #efficientnet #braintumor #mri Moreover, the \(R_B\) parameter has been changed to depend on weibull distribution as described below. & Zhu, Y. Kernel feature selection to fuse multi-spectral mri images for brain tumor segmentation. Mirjalili, S. & Lewis, A. Ge, X.-Y. Nevertheless, a common mistake in COVID-19 dataset fusion, mainly on classification tasks, is that by mixing many datasets of COVID-19 and using as Control images another dataset, there will be . Duan et al.13 applied the Gaussian mixture model (GMM) to extract features from pulmonary nodules from CT images. }\delta (1-\delta ) U_{i}(t-1)+ \frac{1}{3! The MPA starts with the initialization phase and then passing by other three phases with respect to the rational velocity among the prey and the predator. The symbol \(R_B\) refers to Brownian motion. However, using medical imaging, chest CT, and chest X-ray scan can play a critical role in COVID-19 diagnosis. PVT-COV19D: COVID-19 Detection Through Medical Image Classification Using X-ray images we can train a machine learning classifier to detect COVID-19 using Keras and TensorFlow. Fung, G. & Stoeckel, J. Svm feature selection for classification of spect images of alzheimers disease using spatial information. It based on using a deep convolutional neural network (Inception) for extracting features from COVID-19 images, then filtering the resulting features using Marine Predators Algorithm (MPA), enhanced by fractional-order calculus(FO). 35, 1831 (2017). On the second dataset, dataset 2 (Fig. In9, to classify ultrasound medical images, the authors used distance-based FS methods and a Fuzzy Support Vector Machine (FSVM). Finally, the sum of the features importance value on each tree is calculated then divided by the total number of trees as in Eq. J. Clin. While the second half of the agents perform the following equations. These images have been further used for the classification of COVID-19 and non-COVID-19 images using ResNet50 and AlexNet convolutional neural network (CNN) models. Simonyan, K. & Zisserman, A. Appl. Identifying Facemask-Wearing Condition Using Image Super-Resolution BDCC | Free Full-Text | COVID-19 Classification through Deep Learning The proposed approach was evaluated on two public COVID-19 X-ray datasets which achieves both high performance and reduction of computational complexity. Netw. MathSciNet Dhanachandra, N. & Chanu, Y. J. PubMed Isolation and characterization of a bat sars-like coronavirus that uses the ace2 receptor. Automatic segmentation and classification for antinuclear antibody Accordingly, the prey position is upgraded based the following equations. For the exploration stage, the weibull distribution has been applied rather than Brownian to bost the performance of the predator in stage 2 and the prey velocity in stage 1 based on the following formula: Where k, and \(\zeta\) are the scale and shape parameters. The model was developed using Keras library47 with Tensorflow backend48. Aiming at the problems of poor attention to existing translation models, the insufficient ability of key transfer and generation, insufficient quality of generated images, and lack of detailed features, this paper conducts research on lung medical image translation and lung image classification based on . You are using a browser version with limited support for CSS. Lilang Zheng, Jiaxuan Fang, Xiaorun Tang, Hanzhang Li, Jiaxin Fan, Tianyi Wang, Rui Zhou, Zhaoyan Yan. Chowdhury, M.E. etal. Epub 2022 Mar 3. The Softmax activation function is used for this purpose because the output should be binary (positive COVID-19 negative COVID-19). Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Inspired by our recent work38, where VGG-19 besides statistically enhanced Salp Swarm Algorithm was applied to select the best features for White Blood Cell Leukaemia classification. The evaluation showed that the RDFS improved SVM robustness against reconstruction kernel and slice thickness. & Cao, J. Li et al.34 proposed a self-adaptive bat algorithm (BA) to address two problems in lung X-ray images, rebalancing, and feature selection. Using the best performing fine-tuned VGG-16 DTL model, tests were carried out on 470 unlabeled image dataset, which was not used in the model training and validation processes. They concluded that the hybrid method outperformed original fuzzy c-means, and it had less sensitive to noises. Also, some image transformations were applied, such as rotation, horizontal flip, and scaling. Table2 depicts the variation in morphology of the image, lighting, structure, black spaces, shape, and zoom level among the same dataset, as well as with the other dataset. Whereas, the worst algorithm was BPSO. https://doi.org/10.1038/s41598-020-71294-2, DOI: https://doi.org/10.1038/s41598-020-71294-2. Article In this paper, we used TPUs for powerful computation, which is more appropriate for CNN. Also, in12, an Fs method based on SVM was proposed to detect Alzheimers disease from SPECT images. Biomed. Taking into consideration the current spread of COVID-19, we believe that these techniques can be applied as a computer-aided tool for diagnosing this virus. FP (false positives) are the positive COVID-19 images that were incorrectly labeled as negative COVID-19, while FN (false negatives) are the negative COVID-19 images that were mislabeled as positive COVID-19 images. In Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition12511258 (2017). 2022 May;144:105350. doi: 10.1016/j.compbiomed.2022.105350. Huang, P. et al. Google Scholar. The different proposed models will be trained with three-class balanced dataset which consists of 3000 images, 1000 images for each class. where r is the run numbers. The results are the best achieved compared to other CNN architectures and all published works in the same datasets. Then the best solutions are reached which determine the optimal/relevant features that should be used to address the desired output via several performance measures. Hashim, F. A., Houssein, E. H., Mabrouk, M. S., Al-Atabany, W. & Mirjalili, S. Henry gas solubility optimization: a novel physics-based algorithm. Sci. The . Meanwhile, the prey moves effectively based on its memory for the previous events to catch its food, as presented in Eq. Modeling a deep transfer learning framework for the classification of Affectation index and severity degree by COVID-19 in Chest X-ray images Classification Covid-19 X-Ray Images | by Falah Gatea | Medium Building a custom CNN model: Identification of COVID-19 - Analytics Vidhya Mutation: A mutation refers to a single change in a virus's genome (genetic code).Mutations happen frequently, but only sometimes change the characteristics of the virus. A. et al. Duan, H. et al. The Marine Predators Algorithm (MPA)is a recently developed meta-heuristic algorithm that emulates the relation among the prey and predator in nature37. A., Fan, H. & Abd ElAziz, M. Optimization method for forecasting confirmed cases of covid-19 in china. where \(ni_{j}\) is the importance of node j, while \(w_{j}\) refers to the weighted number of samples reaches the node j, also \(C_{j}\) determines the impurity value of node j. left(j) and right(j) are the child nodes from the left split and the right split on node j, respectively. Google Scholar. \end{aligned}$$, $$\begin{aligned} U_i(t+1)-U_i(t)=P.R\bigotimes S_i \end{aligned}$$, $$\begin{aligned} D ^{\delta } \left[ U_{i}(t+1)\right] =P.R\bigotimes S_i \end{aligned}$$, $$D^{\delta } \left[ {U_{i} (t + 1)} \right] = U_{i} (t + 1) + \sum\limits_{{k = 1}}^{m} {\frac{{( - 1)^{k} \Gamma (\delta + 1)U_{i} (t + 1 - k)}}{{\Gamma (k + 1)\Gamma (\delta - k + 1)}}} = P \cdot R \otimes S_{i} .$$, $$\begin{aligned} \begin{aligned} U(t+1)_{i}= - \sum _{k=1}^{m} \frac{(-1)^k\Gamma (\delta +1)U_{i}(t+1-k)}{\Gamma (k+1)\Gamma (\delta -k+1)} + P.R\bigotimes S_i. Article COVID-19 is the most transmissible disease, caused by the SARS-CoV-2 virus that severely infects the lungs and the upper respiratory tract of the human body.This virus badly affected the lives and wellness of millions of people worldwide and spread widely. The parameters of each algorithm are set according to the default values. COVID-19 tests are currently hard to come by there are simply not enough of them and they cannot be manufactured fast enough, which is causing panic. Future Gener. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Moreover, other COVID-19 positive images were added by the Italian Society of Medical and Interventional Radiology (SIRM) COVID-19 Database45. The results of max measure (as in Eq. Comput. This algorithm is tested over a global optimization problem. Classification of Human Monkeypox Disease Using Deep Learning Models Biocybern. Metric learning Metric learning can create a space in which image features within the. Image segmentation is a necessary image processing task that applied to discriminate region of interests (ROIs) from the area of outsides. & Baby, C.J. Emphysema medical image classification using fuzzy decision tree with fuzzy particle swarm optimization clustering. COVID-19 image classification using deep features and fractional-order marine predators algorithm Authors. and pool layers, three fully connected layers, the last one performs classification. It can be concluded that FS methods have proven their advantages in different medical imaging applications19. Comparison with other previous works using accuracy measure. A hybrid learning approach for the stagewise classification and [PDF] COVID-19 Image Data Collection | Semantic Scholar In the meantime, to ensure continued support, we are displaying the site without styles One of the main disadvantages of our approach is that its built basically within two different environments. Also, they require a lot of computational resources (memory & storage) for building & training. The proposed COVID-19 X-ray classification approach starts by applying a CNN (especially, a powerful architecture called Inception which pre-trained on Imagnet dataset) to extract the discriminant features from raw images (with no pre-processing or segmentation) from the dataset that contains positive and negative COVID-19 images. One from the well-know definitions of FC is the Grunwald-Letnikov (GL), which can be mathematically formulated as below40: where \(D^{\delta }(U(t))\) refers to the GL fractional derivative of order \(\delta\). Stage 2: The prey/predator in this stage begin exploiting the best location that detects for their foods. 4b, FO-MPA algorithm selected successfully fewer features than other algorithms, as it selected 130 and 86 features from Dataset 1 and Dataset 2, respectively. First: prey motion based on FC the motion of the prey of Eq. In this paper, we proposed a novel COVID-19 X-ray classification approach, which combines a CNN as a sufficient tool to extract features from COVID-19 X-ray images. 78, 2091320933 (2019). Pangolin - Wikipedia Bibliographic details on CECT: Controllable Ensemble CNN and Transformer for COVID-19 image classification by capturing both local and global image features. As a result, the obtained outcomes outperformed previous works in terms of the models general performance measure. COVID-19 image classification using deep features and fractional-order Among the FS methods, the metaheuristic techniques have been established their performance overall other FS methods when applied to classify medical images. Methods Med. Hence, it was discovered that the VGG-16 based DTL model classified COVID-19 better than the VGG-19 based DTL model. Image Classification With ResNet50 Convolution Neural Network - Medium Expert Syst. Comput. Therefore, in this paper, we propose a hybrid classification approach of COVID-19. & Dai, Q. Discriminative clustering and feature selection for brain mri segmentation. To evaluate the performance of the proposed model, we computed the average of both best values and the worst values (Max) as well as STD and computational time for selecting features. Therefore, reducing the size of the feature from about 51 K as extracted by deep neural networks (Inception) to be 128.5 and 86 in dataset 1 and dataset 2, respectively, after applying FO-MPA algorithm while increasing the general performance can be considered as a good achievement as a machine learning goal. This paper reviews the recent progress of deep learning in COVID-19 images applications from five aspects; Firstly, 33 COVID-19 datasets and data enhancement methods are introduced; Secondly, COVID-19 classification methods . For instance,\(1\times 1\) conv. Types of coronavirus, their symptoms, and treatment - Medical News Today Johnson et al.31 applied the flower pollination algorithm (FPA) to select features from CT images of the lung, to detect lung cancers. It also contributes to minimizing resource consumption which consequently, reduces the processing time. For example, as our input image has the shape \(224 \times 224 \times 3\), Nasnet26 produces 487 K features, Resnet25 and Xception29 produce about 100 K features and Mobilenet27 produces 50 K features, while FO-MPA produces 130 and 86 features for both dataset1 and dataset 2, respectively. In ancient India, according to Aelian, it was . A combination of fractional-order and marine predators algorithm (FO-MPA) is considered an integration among a robust tool in mathematics named fractional-order calculus (FO). Based on54, the later step reduces the memory requirements, and improve the efficiency of the framework. Layers are applied to extract different types of features such as edges, texture, colors, and high-lighted patterns from the images. }, \end{aligned}$$, $$\begin{aligned} D^{\delta }[U(t)]=\frac{1}{T^\delta }\sum _{k=0}^{m} \frac{(-1)^k\Gamma (\delta +1)U(t-kT)}{\Gamma (k+1)\Gamma (\delta -k+1)} \end{aligned}$$, $$\begin{aligned} D^1[U(t)]=U(t+1)-U(t) \end{aligned}$$, $$\begin{aligned} U=Lower+rand_1\times (Upper - Lower ) \end{aligned}$$, $$\begin{aligned} Elite=\left[ \begin{array}{cccc} U_{11}^1&{}U_{12}^1&{}\ldots &{}U_{1d}^1\\ U_{21}^1&{}U_{22}^1&{}\ldots &{}U_{2d}^1\\ \ldots &{}\ldots &{}\ldots &{}\ldots \\ U_{n1}^1&{}U_{n2}^1&{}\ldots &{}U_{nd}^1\\ \end{array}\right] , \, U=\left[ \begin{array}{cccc} U_{11}&{}U_{12}&{}\ldots &{}U_{1d}\\ U_{21}&{}U_{22}&{}\ldots &{}U_{2d}\\ \ldots &{}\ldots &{}\ldots &{}\ldots \\ U_{n1}&{}U_{n2}&{}\ldots &{}U_{nd}\\ \end{array}\right] , \, \end{aligned}$$, $$\begin{aligned} S_i&= {} R_B \bigotimes (Elite_i-R_B\bigotimes U_i), i=1,2,\ldots ,n \end{aligned}$$, $$\begin{aligned} U_i&= {} U_i+P.R\bigotimes S_i \end{aligned}$$, \(\frac{1}{3}t_{max}< t< \frac{2}{3}t_{max}\), $$\begin{aligned} S_i&= {} R_L \bigotimes (Elite_i-R_L\bigotimes U_i), i=1,2,\ldots ,n/2 \end{aligned}$$, $$\begin{aligned} S_i&= {} R_B \bigotimes (R_B \bigotimes Elite_i- U_i), i=1,2,\ldots ,n/2 \end{aligned}$$, $$\begin{aligned} U_i&= {} Elite_i+P.CF\bigotimes S_i,\, CF= \left( 1-\frac{t}{t_{max}} \right) ^{\left(2\frac{t}{t_{max}}\right) } \end{aligned}$$, $$\begin{aligned} S_i&= {} R_L \bigotimes (R_L \bigotimes Elite_i- U_i), i=1,2,\ldots ,n \end{aligned}$$, $$\begin{aligned} U_i&= {} Elite_i+P.CF\bigotimes S_i,\, CF= \left( 1-\frac{t}{t_{max}}\right) ^{\left(2\frac{t}{t_{max}} \right) } \end{aligned}$$, $$\begin{aligned} U_i=\left\{ \begin{array}{ll} U_i+CF [U_{min}+R \bigotimes (U_{max}-U_{min})]\bigotimes W &{} r_5 < FAD \\ U_i+[FAD(1-r)+r](U_{r1}-U_{r2}) &{} r_5 > FAD\\ \end{array}\right. It noted that all produced feature vectors by CNNs used in this paper are at least bigger by more than 300 times compared to that produced by FO-MPA in terms of the size of the featureset. Improving the ranking quality of medical image retrieval using a genetic feature selection method. (22) can be written as follows: By using the discrete form of GL definition of Eq. The MCA-based model is used to process decomposed images for further classification with efficient storage. where \(R_L\) has random numbers that follow Lvy distribution. Chexnet: Radiologist-level pneumonia detection on chest x-rays with deep learning. Also, other recent published works39, who combined a CNN architecture with Weighted Symmetric Uncertainty (WSU) to select optimal features for traffic classification. J. To further analyze the proposed algorithm, we evaluate the selected features by FO-MPA by performing classification. Our proposed approach is called Inception Fractional-order Marine Predators Algorithm (IFM), where we combine Inception (I) with Fractional-order Marine Predators Algorithm (FO-MPA). 97, 849872 (2019). The algorithm combines the assessment of image quality, digital image processing and deep learning for segmentation of the lung tissues and their classification. The whale optimization algorithm. A properly trained CNN requires a lot of data and CPU/GPU time. If the random solution is less than 0.2, it converted to 0 while the random solution becomes 1 when the solutions are greater than 0.2. CAS Multimedia Tools Appl. Decaf: A deep convolutional activation feature for generic visual recognition. Classification of COVID19 using Chest X-ray Images in Keras 4.6 33 ratings Share Offered By In this Guided Project, you will: Learn to Build and Train the Convolutional Neural Network using Keras with Tensorflow as Backend Learn to Visualize Data in Matplotlib Learn to make use of the Trained Model to Predict on a New Set of Data 2 hours [PDF] Detection and Severity Classification of COVID-19 in CT Images Currently, a new coronavirus, called COVID-19, has spread to many countries, with over two million infected people or so-called confirmed cases. Chong, D. Y. et al. Mirjalili, S., Mirjalili, S. M. & Lewis, A. Grey wolf optimizer. The conference was held virtually due to the COVID-19 pandemic. Google Scholar. Article Syst. The predator tries to catch the prey while the prey exploits the locations of its food. Automatic CNN-based Chest X-Ray (CXR) image classification for detecting Covid-19 attracted so much attention. Apostolopoulos, I. D. & Mpesiana, T. A. Covid-19: automatic detection from x-ray images utilizing transfer learning with convolutional neural networks. The focus of this study is to evaluate and examine a set of deep learning transfer learning techniques applied to chest radiograph images for the classification of COVID-19, normal (healthy), and pneumonia. The proposed approach selected successfully 130 and 86 out of 51 K features extracted by inception from dataset 1 and dataset 2, while improving classification accuracy at the same time. So, there might be sometimes some conflict issues regarding the features vector file types or issues related to storage capacity and file transferring. The following stage was to apply Delta variants. & Mirjalili, S. Slime mould algorithm: A new method for stochastic optimization. The results are the best achieved on these datasets when compared to a set of recent feature selection algorithms. Arjun Sarkar - Doctoral Researcher - Leibniz Institute for Natural Automatic diagnosis of COVID-19 with MCA-inspired TQWT-based In this paper, a new ML-method proposed to classify the chest x-ray images into two classes, COVID-19 patient or non-COVID-19 person. Kharrat, A. (5). \(Fit_i\) denotes a fitness function value. Design incremental data augmentation strategy for COVID-19 CT data. In this paper, after applying Chi-square, the feature vector is minimized for both datasets from 51200 to 2000. J. et al. This stage can be mathematically implemented as below: In Eq. arXiv preprint arXiv:2004.07054 (2020). While the second dataset, dataset 2 was collected by a team of researchers from Qatar University in Qatar and the University of Dhaka in Bangladesh along with collaborators from Pakistan and Malaysia medical doctors44. Sahlol, A.T., Yousri, D., Ewees, A.A. et al. Classification of COVID-19 X-ray images with Keras and its - Medium \end{aligned} \end{aligned}$$, $$\begin{aligned} WF(x)=\exp ^{\left( {\frac{x}{k}}\right) ^\zeta } \end{aligned}$$, $$\begin{aligned}&Accuracy = \frac{\text {TP} + \text {TN}}{\text {TP} + \text {TN} + \text {FP} + \text {FN}} \end{aligned}$$, $$\begin{aligned}&Sensitivity = \frac{\text {TP}}{\text{ TP } + \text {FN}}\end{aligned}$$, $$\begin{aligned}&Specificity = \frac{\text {TN}}{\text {TN} + \text {FP}}\end{aligned}$$, $$\begin{aligned}&F_{Score} = 2\times \frac{\text {Specificity} \times \text {Sensitivity}}{\text {Specificity} + \text {Sensitivity}} \end{aligned}$$, $$\begin{aligned} Best_{acc} = \max _{1 \le i\le {r}} Accuracy \end{aligned}$$, $$\begin{aligned} Best_{Fit_i} = \min _{1 \le i\le r} Fit_i \end{aligned}$$, $$\begin{aligned} Max_{Fit_i} = \max _{1 \le i\le r} Fit_i \end{aligned}$$, $$\begin{aligned} \mu = \frac{1}{r} \sum _{i=1}^N Fit_i \end{aligned}$$, $$\begin{aligned} STD = \sqrt{\frac{1}{r-1}\sum _{i=1}^{r}{(Fit_i-\mu )^2}} \end{aligned}$$, https://doi.org/10.1038/s41598-020-71294-2. }\delta (1-\delta )(2-\delta )(3-\delta ) U_{i}(t-3) + P.R\bigotimes S_i. Abbas, A., Abdelsamea, M.M. & Gaber, M.M. Classification of covid-19 in chest x-ray images using detrac deep convolutional neural network. So, for a \(4 \times 4\) matrix, will result in \(2 \times 2\) matrix after applying max pooling. CNNs are more appropriate for large datasets. Besides, the used statistical operations improve the performance of the FO-MPA algorithm because it supports the algorithm in selecting only the most important and relevant features. Future Gener. In this subsection, a comparison with relevant works is discussed. (14)(15) to emulate the motion of the first half of the population (prey) and Eqs. However, some of the extracted features by CNN might not be sufficient, which may affect negatively the quality of the classification images. For diagnosing COVID-19, the RT-PCR (real-time polymerase chain reaction) is a standard diagnostic test, but, it can be considered as a time-consuming test, more so, it also suffers from false negative diagnosing4.